indelG: Peach vs Nectarine
A peach is a peach is a peach. Or is it a nectarine? A common question the public asks peach breeders and producers is: What is the difference between a peach and a nectarine? The prevalence of this question illustrates the importance of pubescence and the selection for or against “fuzz” in commercial breeding programs. Simply put, a nectarine is a fuzz-free peach.
Genetics
The presence or absence of skin fuzz is controlled by a single dominant allele associated with the G locus."The nectarine-type allele is recessive to the peach-type. Hidden within many peach cultivars is the genetic potential to produce nectarine offspring—because those cultivars are heterozygous. Although other attributes, such as “nectarine flavor”, are found in nectarine cultivar descriptions, this may be due to other loci, not pleiotropy at the G locus, as breeders are able to develop peach cultivars with the “nectarine flavor”. To predict if an individual will be a nectarine or a peach and if a peach has the genetic potential to produce nectarine offspring, the indelG DNA test was developed.* The indelG DNA test has two alleles: “P”, the presence of which always results in a peach, and “n”, which must be homozygous to result in a nectarine. Both alleles are common in U.S. breeding germplasm.
Predictive Capacity
IndelG is 100% predictive. Nectarine homozygotes will always lack pubescence. Heterozygotes will always have pubescence, but can produce nectarine offspring. Peach homozygotes will always have pubescence. Confirm the effects in your own germplasm before widespread use. The indelG DNA test can be useful in parent selection for eliminating or maximizing the possibility of nectarine seedlings. This simple PCR-based test consists of a single primer pair that can be run on a variety of platforms including agarose gels.
When to Assay
The indelG DNA test can be useful in parent selection for eliminating or maximizing the possibility of nectarine seedlings.
Allelic State of Several Peach & Nectarine Cultivars
Genotype | Example Cultivars | Phenotype |
---|---|---|
P|P | Elberta, Contender | peach |
P|n | O’Henry, Automnglo | peach |
n|n | all nectarine cultivars | nectarine |
A table of haplotypes for important U.S. peach germplasm can be downloaded here.
Alleles Available
The indelG DNA test has two alleles: “P”, the presence of which always results in a peach, and “n”, which must be homozygous to result in a nectarine. Both alleles are common in U.S. breeding germplasm.
Technical Details
This simple PCR-based test consists of a single primer pair that can be run on a variety of platforms including agarose gels.